產品貨號 :  MLR-0647

英文名稱 :  CD4

中文名稱 :  CD4抗體

別    名 :  CD4 (L3T4); CD4 antigen (p55); CD4 Antigen ; CD4 molecule; CD4 Receptor; CD4+ Lymphocyte deficiency, included; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; p55; T Cell Antigen T4 ; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3 ; T cell surface antigen T4/Leu3; T Cell Surface Glycoprotein CD4; W3/25; W3/25 antigen; T-cell surface glycoprotein CD4 isoform 1 precursor; CD4_HUMAN.  

研究領域 :  細胞生物 腫瘤 免疫學  干細胞  轉錄調節因子  細胞表面分子  t-淋巴細胞  

抗體來源 :  Rabbit

克隆類型 :  Polyclonal

交叉反應 :  Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig, MACFA

產品應用 : WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  48kDa

細胞定位 :  細胞膜

性    狀 :  Lyophilized or Liquid

濃    度 :  1mg/ml

免 疫 原:  KLH conjugated synthetic peptide derived from human CD4:385-457/457

亞    型 :  IgG

純化方法 :  affinity purified by Protein A

儲 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

產品介紹background :

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010].

Function:

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

Subunit:

Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Subcellular Location:

Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.

Post-translational modifications:

Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

Similarity:

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:

P01730

Gene ID:

920

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

此抗體可識別55KDⅠ型單鏈穿膜糖蛋白。 CD4分子是存在于大多數輔助/誘導T細胞表面的59kDa的糖蛋白。正常淋巴組織中CD4的表達數量多于CD8，此抗體主要用于標記輔助/誘導T細胞，與CD8單抗聯合使用對外周血淋巴細胞分型。 CD4抗原是HLA-II類分子和人類免疫缺陷病毒(HIV)-愛滋病的受體，在35-50%外周血淋巴細胞-輔助和誘導T細胞(Th/Ti)和70-80%人胸腺細胞上表達，在人的單核細胞表面也有低密度的表達。 CD4抗原有膜結合型和可溶性兩種形式。Th/Ti可輔助Ig產生和T細胞毒T細胞的作用。

產品圖片: