產品貨號 :  mlR3884

英文名稱 :  GPX4

中文名稱 :  谷胱甘肽過氧化酶4抗體

別    名 :  Glutathione peroxidase 4; GPX 4; GPX-4; GPX4; GPX4_HUMAN; GSHPx-4; MCSP; mitochondrial; PHGPx; Phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase mitochondrial; snGPx; snPHGPx; Sperm nucleus glutathione peroxidase.  

研究領域 :  腫瘤  細胞生物  免疫學  信號轉導  新陳代謝  線粒體  

抗體來源 :  Rabbit

克隆類型 :  Polyclonal

交叉反應 :   Human, Mouse, Rat, Pig, Cow,

產品應用 :  WB=1:500-2000 ELISA=1:500-1000  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  22kDa

細胞定位 :  細胞漿 線粒體

性    狀 :  Lyophilized or Liquid

濃    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human Glutathione Peroxidase 4:101-197/197

亞    型 :  IgG

純化方法 :  affinity purified by Protein A

儲 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

產品介紹background:

Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.

Function:

Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.

Subunit:

Monomer. Has a tendency to form higher mass oligomers.

Subcellular Location:

Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm.

Tissue Specificity:

Present primarily in testis.

Similarity:

Belongs to the glutathione peroxidase family.

SWISS:

P36969

Gene ID:

2879

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

谷胱甘肽過氧化物酶（GSH-Px）是機體內廣泛存在的一種重要的過氧化物分解酶。硒是GSH-Px酶系的組成成分，它能催化GSH變為GSSG，使有毒的過氧化物還原成無毒的羥基化合物，同時促進H2O2的分解，從而保護細胞膜的結構及功能不受過氧化物的干擾及損害。GSH-Px的活性中心是硒半胱氨酸，其活力大小可以反映機體硒水平。GSH-Px酶系主要包括4種不同的GSH-Px，分別為胞漿GSH-Px、血漿GSH-Px、磷脂氫過氧化物GSH-Px及胃腸道專屬性GSH-Px。胞漿GSH-Px由4個相同的分子量大小為22kDa的亞基構成四聚體，每個亞基含有1個分子硒半胱氨酸，廣泛存在于機體內各個組織，以肝臟紅細胞為最多。它的生理功能主要是催化GSH參與過氧化反應，清除在細胞呼吸代謝過程中產生的過氧化物和羥自由基，從而減輕細胞膜多不飽和脂肪酸的過氧化作用。