產(chǎn)品貨號 :  mlR33324

英文名稱 :  Beclin 1

中文名稱 :  Bcl-2同源結(jié)構(gòu)域蛋白單克隆抗體

別    名 :  Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like protein 1; 4921513J16Rik; 5430417M23Rik; ATG6; BECLIN 1; BECN1; MGC6843; BECN1_HUMAN.  

研究領(lǐng)域 :  腫瘤  免疫學  神經(jīng)生物學  細胞凋亡  

抗體來源 :  Mouse

克隆類型 :  Monoclonal

克 隆 號 :  7D7

交叉反應 :   Human, Mouse, Rat,

產(chǎn)品應用 :  IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  50kDa

細胞定位 :  細胞漿 細胞膜

性    狀 :  Lyophilized or Liquid

濃    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human Beclin 1:

亞    型 :  IgG

純化方法 :  affinity purified by Protein G

儲 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

產(chǎn)品介紹  :  Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 52kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons.

Function:

Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

Subunit:

Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB. Interacts with DAPK1. Interacts with human cytomegalovirus/HHV-5 protein TRS1.

Subcellular Location:

Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.

Tissue Specificity:

Ubiquitous.

Post-translational modifications:

Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.

Ubiquitinated. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes.

Similarity:

Belongs to the beclin family.

SWISS:

Q14457

Gene ID:

8678

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

BECN1是一種分子質(zhì)量為60KDa的蛋白質(zhì), 它能與細胞凋亡抑制劑Bcl-2相互作用, 減少中樞神經(jīng)系統(tǒng)的凋亡，參與PI3K復合物的組成和自噬體的形成。主要表達在胞質(zhì)。抑癌基因beclin 1通過自噬作用來調(diào)節(jié)細胞生長。

產(chǎn)品圖片