產品貨號 :  mlR23835

英文名稱 :  ZO-1

中文名稱 :  胞質緊密粘連蛋白1/閉鎖小帶蛋白1抗體

別    名  :  ZO1 tight junction protein; Tight junction protein 1; Tight junction protein ZO-1; Tight junction protein ZO1; TJP1; zo-1; Zo1; ZO1_HUMAN; Zona occludens 1; Zona occludens 1 protein; Zona occludens protein 1; Zonula occludens 1 protein; Zonula occludens protein 1.  

研究領域 :  細胞生物  免疫學  信號轉導  細胞粘附分子  細胞表面分子  

抗體來源 :  Rabbit

克隆類型 :  Polyclonal

交叉反應  :  Human, Mouse, Rat, Dog, Cow, Rabbit,

產品應用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  191kDa

細胞定位 :  細胞漿 細胞膜

性    狀 :  Lyophilized or Liquid

濃    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human ZO-1 :1501-1600/1748

亞    型 :  IgG

純化方法 :  affinity purified by Protein A

儲 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

產品介紹 :   This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.

Function:

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

Subunit:

Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with OCLN, CALM, claudins, CGN/cingulin, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with CDC42BPB and MYZAP. Interacts (via PDZ domain) with GJA1.

Subcellular Location:

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction. Cell junction, gap junction. Note=Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells.

Tissue Specificity:

The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Post-translational modifications:

Phosphorylated. Dephosphorylated by PTPRJ.

Similarity:

Belongs to the MAGUK family.

Contains 1 guanylate kinase-like domain.

Contains 3 PDZ (DHR) domains.

SWISS:

Q07157

Gene ID:

7082

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

胞質緊密粘連蛋白1（ZO-1）是多結構域蛋白家族膜結合鳥苷酸激酶的家族成員，在緊密連接蛋白的組成成分中起到對組織分化和器官形成方面起較重要的作用。ZO-1在包括腎、胎盤、血腦屏障等許多組織都有不同的表達，可與緊密連接上的很多跨膜蛋白相互作用。也有學者認為：ZO-1的改變與細胞通透性的增加有關。

產品圖片