產品貨號 : mlR5605
英文名稱 : phospho-TERT (Ser1125)
中文名稱 : 磷酸化端粒酶逆轉錄酶抗體
別 名 : p-TERT(Ser1125); TERT(phospho Ser1125); TERT(phospho S1125); EST2; hEST2; TCS1; Telomerase associated protein 2; Telomerase Catalytic Subunit; Telomere Reverse Transcriptase; TERT; TP2; TRT.
產品類型 : 磷酸化抗體
研究領域 : 腫瘤 免疫學 細胞凋亡 轉錄調節因子
抗體來源 : Rabbit
克隆類型 : Polyclonal
交叉反應 : Human, Mouse, Rat,
產品應用 : WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=0.2μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 : 124kDa
細胞定位 : 細胞核
性 狀 : Lyophilized or Liquid
濃 度 : 1mg/ml
免 疫 原 : KLH conjugated Synthesised phosphopeptide derived from human TERT around the phosphorylation site of Ser1125:LP(p-S)DF
亞 型 : IgG
純化方法 : affinity purified by Protein A
儲 存 液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
產品介紹background:
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Telomerase are large DNA-protein complexes with telomerase expression being the subject of recent research due to its link to cell immortalization. Recent evidence has shown that MYC upregulates the catalytic subunit of telomerase, TERT, and that TERT cooperates with HPV E7 in cell immortalization. Ever since the discovery that telomeres are short in cancer cells and telomerase is activated in immortal cells, telomerase has been associated with oncogenes. During the past year, major advances have been made in understanding the link between telomerase expression and cell immortality. Studies of yeast telomeres have revealed an unexpected role for the non-homologous end-joining machinery in telomere maintenance and have provided the first definitive evidence that telomeres play a critical role in meiosis. Identification of new telomere proteins has led to a better understanding of vertebrate telomere structure and function.
SWISS:
O14746
Gene ID:
7015
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
端粒酶逆轉錄酶hTERT是構成端粒酶的組分之一,是端粒酶活性的必需和限速成分,其水平決定細胞端粒酶的活性.抑制hTERT可降低端粒酶的活性,從而抑制瘤細胞生長.目前對hTERT的研究已成為端粒酶研究的熱點問題,已發現TERT蛋白表達在腫瘤診斷中有重要意義,并制備了hTERT抗體及應用核酶技術等來抑制hTERT蛋白的表達,抑制端粒酶活性,從而抑制腫瘤的生長.
端粒反轉錄酶又稱端粒酶催化亞單位 （hTRT；Telomerase catalytic subunit；HEST2；Telomerase-associated protein 2；TP2；Telomerase reverse transcriptase；telomerase catalytic subunit）是細胞永生化及惡性腫瘤發生過程中的端粒酶活化的主要限速步驟。hTRT基因表達可以反映端粒酶活性，與端粒酶活性具有平行關系.
產品圖片
