產品貨號 :  mlR7717

英文名稱 :  CDCA5

中文名稱 :  細胞分裂周期相關蛋白5抗體

別    名 :  Cell division cycle associated protein 5; MGC16386; p35; Sororin; CDCA5_HUMAN.  

研究領域 :  細胞生物  染色質和核信號  細胞周期蛋白  細胞分化  表觀遺傳學  

抗體來源 :  Rabbit

克隆類型 :  Polyclonal

交叉反應  :  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,

產品應用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蠟切片需做抗原修復）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  28kDa

細胞定位 :  細胞核

性    狀 :  Lyophilized or Liquid

濃    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human CDCA5:101-200/252

亞    型 :  IgG

純化方法 :  affinity purified by Protein A

儲 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

產品介紹 :   Sororin, also designated cell division cycle-associated protein 5 (CDCA5) or p35, functions as a regulator of sister chromatid cohesion during mitosis. It interacts with the APC/C complex and is found in a complex consisting of cohesion components SCC-112, MC1L1, SMC3L1, RAD21 and APRIN. The deduced human and mouse Sororin proteins consist of 252 and 264 amino acid residues, respectively, and both contain a KEN box for APC-dependent ubiquitination. Reserach demonstrates a punctated nuclear distribution of Sororin during interphase and a diffuse distribution throughout the cell during mitosis. There is no apparent concentration of Sororin on chromatin in mitotic cells and Sororin levels decrease in synchronized HeLa cells during interphase. The Sororin gene maps to chromosome 11q12.1.

Function:

CDCA5 is a regulator of sister chromatid cohesion in mitosis. It may act by regulating the ability of the cohesin complex to mediate sister chromatid cohesion, perhaps by altering the nature of the interaction of cohesin with the chromosomes. CDCA5 interacts with the APC/C complex and is found in a complex with the cohesin components SMC1L1, SMC3L1, RAD21, PDS5A/APRIN and PDS5B/SCC-112.

Subunit:

Interacts with the APC/C complex (By similarity). Interacts with the chromatin-bound cohesin complex; the interaction is indirect, occurs after DNA replication and requires acetylation of the cohesin component SMC3. Interacts (via the FGF motif) with PDS5A and PDS5B; the interaction is direct and prevents the interaction of PDS5A with WAPAL.

Subcellular Location:

Nuclear.

Post-translational modifications:

Phosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5.

Ubiquitinated by the APC/C complex in G1, leading to its degradation (Probable).

Similarity:

Belongs to the sororin family.

SWISS:

Q96FF9

Gene ID:

113130

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

產品圖片